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KMID : 0191120140290040556
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Journal of Korean Medical Science
2014 Volume.29 No. 4 p.556 ~ p.563
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Effects of Transglutaminase 2 Inhibition on Ventilator-Induced Lung Injury
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Suh In-Bum
Yoon Dae-Wui
Oh Won-Oak
Lee Eun-Joo
Min Kyung-Hoon
Hur Gyu-Young
Lee Seung-Heon
Lee Sung-Yong
Lee Sang-Yeub
Shin Chol
Shim Jae-Jeong
In Kwang-Ho
Kang Kyung-Ho
Kim Je-Hyeong
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Abstract
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This study was performed to examine the role of transglutaminase 2 (TG2) in ventilator-induced lung injury (VILI). C57BL/6 mice were divided into six experimental groups: 1) control group; 2) lipopolysaccharide (LPS) group; 3) lung protective ventilation (LPV) group; 4) VILI group; 5) VILI with cystamine, a TG2 inhibitor, pretreatment (Cyst+VILI) group; and 6) LPV with cystamine pretreatment (Cyst+LPV) group. Acute lung injury (ALI) score, TG2 activity and gene expression, inflammatory cytokines, and nuclear factor-κB (NF-κB) activity were measured. TG2 activity and gene expression were significantly increased in the VILI group (P < 0.05). Cystamine pretreatment significantly decreased TG2 activity and gene expression in the Cyst+VILI group (P < 0.05). Inflammatory cytokines were higher in the VILI group than in the LPS and LPV groups (P < 0.05), and significantly lower in the Cyst+VILI group than the VILI group (P < 0.05). NF-κB activity was increased in the VILI group compared with the LPS and LPV groups (P < 0.05), and significantly decreased in the Cyst+VILI group compared to the VILI group (P = 0.029). The ALI score of the Cyst+VILI group was lower than the VILI group, but the difference was not statistically significant (P = 0.105). These results suggest potential roles of TG2 in the pathogenesis of VILI.
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KEYWORD
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Acute Lung Injury, Respiratory Distress Syndrome, Adult, Respiration, Artificial, Ventilator-Induced Lung Injury, Inflammation, Transglutaminase 2
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